Recombinant Caldesmon HMW antibody (CF®488A)

Catalog No. ABIN6162481

The Rabbit Monoclonal anti- antibody has been validated for FACS, IF and IHC. It is suitable to detect in samples from Human and Rat.

Quick Overview for Recombinant Caldesmon HMW antibody (CF®488A) (ABIN6162481)

Target: Caldesmon HMW

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: CALD1-1424R

Product Details

Purpose: Recombinant Rabbit Monoclonal anti-Caldesmon, HMW (CALD1/1424R), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CALD1 protein

Isotype: IgG kappa

Application Details

Application Notes: Immunohistology (formalin) 1-2 μg/mL

• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA
•   pH 7.5-8.5
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: Caldesmon HMW

Alternative Name: Caldesmon, HMW

Background: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Molecular Weight: 150 kDa

Gene ID: 800, 490203

UniProt: Q05682